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"count": 43797,
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"results": [
{
"accession": "EFO:0002624",
"name": "spinocerebellar ataxia",
"definition": "['A group of dominantly inherited, predominatly late-onset, cerebellar ataxias which have been divided into multiple subtypes based on clinical features and genetic mapping. Progressive ataxia is a central feature of these conditions, and in certain subtypes POLYNEUROPATHY; DYSARTHRIA; visual loss; and other disorders may develop (MeSH).']",
"term_type": "cell line"
},
{
"accession": "EFO:0002970",
"name": "myopathy",
"definition": "[]",
"term_type": "cell line"
},
{
"accession": "EFO:0004185",
"name": "enrichment of methylated DNA",
"definition": "[\"The isolation and enrichment of methylated DNA by using methyl CpG binding proteins, MBD2b protein in particular.\\n\\nMethylation of cytosines located 5' to guanosine is known to have a profound effect on the expression of many eukaryotic genes. In normal cells methylation occurs predominantly in CG-poor regions, while CG-rich areas, called CpG-islands remain unmethylated. The exceptions are the extensive methylation of CpG islands associated with transcriptional inactivation of regulatory regions of imprinted genes and genes on the inactive X-chromosome of females. Aberrant methylation of normally unmethylated CpG islands has been documented as a relatively frequent event in immortalized and transformed cells and has been associated with transcriptional inactivation of defined tumor suppressor genes in human cancers.\\n\\nTo evalute the methylation status of either a specific locus or an entire genome, the isolation and enrichment of methylated DNA can be a useful first step. A high-affinity GST-MBD protein pre-bound to a magnetic bead is used to enable this enrichement (Fraga M.F., et al. (2003). Nuc. Acids Res, 31, 1765–1774).\"]",
"term_type": "Enrichment process"
},
{
"accession": "EFO:0009106",
"name": "sample enrichment",
"definition": "['process which results in the increased concentration of an entity of interest, such as a particle or cell, in a sample']",
"term_type": "Enrichment process"
},
{
"accession": "EFO:0009108",
"name": "fluorescence-activated cell sorting",
"definition": "['A flow cytometry assay that provides a method for sorting a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell.\\nThe cells are suspended in a stream of fluid and forced individually through a vibrating nozzle, then exposed to a laser beam and the resulting fluorescence and scattered light is detected. Finally the cells are sorted by applying an electrical charge to droplets of the fluid and deflecting it to the left or right using charged electrodes.']",
"term_type": "Enrichment process"
},
{
"accession": "EFO:0009109",
"name": "magnetic affinity cell sorting",
"definition": "['A method of cell sorting that uses paramagnetic beads that are coated with antibodies, antigens, receptors, receptor substrates, binding proteins, etc to separate target cells from solution.']",
"term_type": "Enrichment process"
},
{
"accession": "EFO:0009110",
"name": "Ficoll-Hypaque method",
"definition": "['A centrifugation procedure that utilizes a density-gradient medium to separate mononuclear and polymorphonuclear leukocytes from other formed elements in the blood.']",
"term_type": "Enrichment process"
},
{
"accession": "EFO:0009111",
"name": "laser capture microdissection",
"definition": "['Laser capture microdissection is a technique used for isolating specific cells of interest from microscopic regions of tissue/cells/organisms.\\nIt allows sampling of specific cells under direct microscopic visualization. A film is placed against the heterogeneous tissue and activated by a laser beam to capture only the cells of interest. Those cells are transferred to the film to provide a visual microscopic record of what was transferred, which can be analyzed for DNA, RNA, or protein. The film upon which the cells are transferred is incorporated into the cap of a vial so that, when the transfer is done under a routine microscopic visualization, the microdissected material can immediately be put into a vial for processing. The system is integrated into a microscope so that the cells are microdissected, transferred to the cap, and then rotated into a vial by a rotating arm in a hands-off operation. (from Cancer Genome Anatomy Project (CGAP) Update) [ NCI ]']",
"term_type": "Enrichment process"
},
{
"accession": "EFO:0009112",
"name": "density gradient centrifugation",
"definition": "['A method of separating cells in a sample by either their differential rate of sedimentation in a centrifugal gradient or their differential buoyancy in a density gradient.']",
"term_type": "Enrichment process"
},
{
"accession": "EFO:0009337",
"name": "cell size selection",
"definition": "['filtering process that either includes or excludes cells of a certain diameter using a physical filter']",
"term_type": "Enrichment process"
},
{
"accession": "EFO:0010048",
"name": "Fluidigm C1-based dissociation",
"definition": "['Dissociation of a sample into individual cells using the Fluidigm C1 platform. Cells are captured on the C1 system (Fluidigm) and processed using the SMARTer chemistry (Clontech) according to the Fluidigm protocol']",
"term_type": "Enrichment process"
},
{
"accession": "EFO:0010175",
"name": "EasySep cell separation",
"definition": "['Cells are targeted for either removal (negative selection and depletion) or selection (positive selection) using antibody complexes directed to specific cell surface antigens. The antibody complexes link targeted cells to EasySep™ magnetic particles. Labeled cells are pulled to the sides of the tube when the sample is placed in an EasySep™ magnet. Magnetically labeled cells will remain in the tube while the untouched cells can be simply poured or pipetted off into a new tube.']",
"term_type": "Enrichment process"
},
{
"accession": "EFO:0010180",
"name": "droplet-based cell isolation",
"definition": "['']",
"term_type": "Enrichment process"
},
{
"accession": "EFO:0010181",
"name": "cell picking",
"definition": "['A manual method to select live single cells from a cell suspension, tissue or whole organism.']",
"term_type": "Enrichment process"
},
{
"accession": "EFO:0010182",
"name": "mouth pipetting",
"definition": "[\"A method of using the researcher's mouth to apply small negative pressure to aspirate a volume into a pipette and then release it into a test tube. This technique can be used to isolate live single cells for single-cell analysis.\"]",
"term_type": "Enrichment process"
},
{
"accession": "EFO:0010806",
"name": "enrichment of phosphorylated protein",
"definition": "['The enrichment of phosphorylation in proteins using different methods such as the use of titanium dioxide (TiO2).']",
"term_type": "Enrichment process"
},
{
"accession": "GO:0000003",
"name": "reproduction",
"definition": "['The production of new individuals that contain some portion of genetic material inherited from one or more parent organisms.']",
"term_type": "cell line"
},
{
"accession": "GO:0000226",
"name": "microtubule cytoskeleton organization",
"definition": "['A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising microtubules and their associated proteins.']",
"term_type": "cell line"
},
{
"accession": "GO:0000271",
"name": "polysaccharide biosynthetic process",
"definition": "['The chemical reactions and pathways resulting in the formation of a polysaccharide, a polymer of many (typically more than 10) monosaccharide residues linked glycosidically.']",
"term_type": "cell line"
},
{
"accession": "GO:0000272",
"name": "polysaccharide catabolic process",
"definition": "['The chemical reactions and pathways resulting in the breakdown of a polysaccharide, a polymer of many (typically more than 10) monosaccharide residues linked glycosidically.']",
"term_type": "cell line"
}
]
}